HZI

Dr. Robert Geffers
0531 6181 3058
E-mail verfassen

Second generation sequencer

Sequenziermodus

Sequencing by synthesis

167 bis 6000

Laufzeit [h]

16 bis 44

• mRNA sequencing
• totalRNA sequencing
• smallRNA sequencing
• microRNA sequencing
• ChIP sequencing
• CLIP-; Ribo-; sequencing (und mehr)
• target enrichment sequencing
• amplicon sequencing
• exome sequencing
• whole genome sequencing

• 1x 50bp single reads, 1,5 billion cluster (S1), 3 billion cluster (S2), 9 billion cluster (S4)
• 1x 100bp single reads, 1,5 billion cluster (S1), 3 billion cluster (S2), 9 billion cluster (S4)
• 1x 150bp single reads, 1,5 billion cluster (S1), 3 billion cluster (S2), 9 billion cluster (S4)
• 2x 50bp paired-end reads, 1,5 billion cluster (S1), 3 billion cluster (S2), 9 billion cluster (S4)
• 2x 100bp paired-end reads, 1,5 billion cluster (S1), 3 billion cluster (S2), 9 billion cluster (S4)
• 2x 150bp paired-end reads, 1,5 billion cluster (S1), 3 billion cluster (S2), 9 billion cluster (S4)

HZI

Dr. Robert Geffers
0531 6181 3058
E-mail verfassen

Second generation sequencer

Sequenziermodus

Sequencing by synthesis

9 bis 1000

Laufzeit [h]

7 bis 144

• mRNA sequencing
• totalRNA sequencing
• smallRNA sequencing
• microRNA sequencing
• ChIP sequencing
• CLIP-; Ribo-; sequencing (und mehr)
• target enrichment sequencing
• amplicon sequencing
• exome sequencing
• whole genome sequencing

Research Core Unit Genomics (RCUG)

Dr. Oliver Dittrich-Breiholz
0511 532 5814
E-mail verfassen

Second generation sequencer

Sequenziermodus

Sequencing by synthesis

16 bis 120

Laufzeit [h]

11 bis 29

• mRNA sequencing
• totalRNA sequencing
• smallRNA sequencing
• microRNA sequencing
• ChIP sequencing
• CLIP-; Ribo-; sequencing (und mehr)
• target enrichment sequencing
• amplicon sequencing
• exome sequencing (eingeschränkt)
• whole genome sequencing (eingeschränkt)

• 1x 75bp single reads, 400 million cluster
• 2x 75bp paired-end reads, 400 million cluster
• 2x 150bp paired-end reads, 400 million cluster
• 2x 75bp paired-end reads, 130 million cluster
• 2x 150bp paired-end reads, 130 million cluster

DSMZ

Dr. Boyke Bunk
0531 2616 322
E-mail verfassen

Second generation sequencer

Sequenziermodus

Sequencing by synthesis

16 bis 120

Laufzeit [h]

11 bis 29

• mRNA sequencing
• totalRNA sequencing
• smallRNA sequencing
• microRNA sequencing
• ChIP sequencing
• CLIP-; Ribo-; sequencing (und mehr)
• target enrichment sequencing
• amplicon sequencing
• exome sequencing (eingeschränkt)
• whole genome sequencing (eingeschränkt)

• 1x 75bp single reads, 400 million cluster
• 2x 75bp paired-end reads, 400 million cluster
• 2x 150bp paired-end reads, 400 million cluster
• 2x 75bp paired-end reads, 130 million cluster
• 2x 150bp paired-end reads, 130 million cluster

Research Core Unit Genomics (RCUG)

Dr. Oliver Dittrich-Breiholz
0511 532 5814
E-mail verfassen

Second generation sequencer

Sequenziermodus

Sequencing by synthesis

16 bis 120

Laufzeit [h]

11 bis 29

• mRNA sequencing
• totalRNA sequencing
• smallRNA sequencing
• microRNA sequencing
• ChIP sequencing
• CLIP-; Ribo-; sequencing (und mehr)
• target enrichment sequencing
• amplicon sequencing
• exome sequencing (eingeschränkt)
• whole genome sequencing (eingeschränkt)

• 1x 75bp single reads, 400 million cluster
• 2x 75bp paired-end reads, 400 million cluster
• 2x 150bp paired-end reads, 400 million cluster
• 2x 75bp paired-end reads, 130 million cluster
• 2x 150bp paired-end reads, 130 million cluster

DSMZ

Dr. Boyke Bunk
0531 2616 322
E-mail verfassen

Second generation sequencer

Sequenziermodus

Sequencing by synthesis

0,54 bis 15

Laufzeit [h]

4 bis 56

• small genone sequencing
• small transcriptome sequencing
• microRNA sequencing
• target enrichment sequencing
• amplicon sequencing

• 1x 36bp single reads, 12-15 million cluster (v2)
• 2x 25bp paired-end reads, 12-15 million cluster (v2)
• 2x 150bp paired-end reads, 12-15 million cluster (v2)
• 2x 250bp paired-end reads, 12-15 million cluster (v2)
• 2x 75bp paired-end reads, 22-25 million cluster (v3)
• 2x 300bp paired-end reads, 22-25 million cluster (v3)

HZI

Dr. Robert Geffers
0531 6181 3058
E-mail verfassen

Second generation sequencer

Sequenziermodus

Sequencing by synthesis

0,54 bis 15

Laufzeit [h]

4 bis 56

• small genone sequencing
• small transcriptome sequencing
• microRNA sequencing
• target enrichment sequencing
• amplicon sequencing

• 1x 36bp single reads, 12-15 million cluster (v2)
• 2x 25bp paired-end reads, 12-15 million cluster (v2)
• 2x 150bp paired-end reads, 12-15 million cluster (v2)
• 2x 250bp paired-end reads, 12-15 million cluster (v2)
• 2x 75bp paired-end reads, 22-25 million cluster (v3)
• 2x 300bp paired-end reads, 22-25 million cluster (v3)

Research Core Unit Genomics (RCUG)

Dr. Oliver Dittrich-Breiholz
0511 532 5814
E-mail verfassen

Second generation sequencer

Sequenziermodus

Sequencing by synthesis

0,54 bis 15

Laufzeit [h]

4 bis 56

• small genone sequencing
• small transcriptome sequencing
• microRNA sequencing
• target enrichment sequencing
• amplicon sequencing

• 1x 36bp single reads, 12-15 million cluster (v2)
• 2x 25bp paired-end reads, 12-15 million cluster (v2)
• 2x 150bp paired-end reads, 12-15 million cluster (v2)
• 2x 250bp paired-end reads, 12-15 million cluster (v2)
• 2x 75bp paired-end reads, 22-25 million cluster (v3)
• 2x 300bp paired-end reads, 22-25 million cluster (v3)

DSMZ

Dr. Boyke Bunk
0531 2616 322
E-mail verfassen

Third generation sequencer

Sequenziermodus

Single molecule sequencing

3 bis 7

Laufzeit [h]

0,5 bis 10

• whole genome sequencing
• long read sequencing
• 16S rRNA gene amplicon sequencing
• epigenetic modifications
• isoform sequencing (RNA Seq)
• targeted sequencing

• 30 min
• 120 min
• 240 min
• 360 min
• 480 min
• 600 min

NIFE

Dr. Sascha Nico Stumpp
0511 532 1419
E-mail verfassen

Third generation sequencer

Sequenziermodus

Single molecule sequencing

3 bis 7

Laufzeit [h]

0,5 bis 10

• whole genome sequencing
• long read sequencing
• 16S rRNA gene amplicon sequencing
• epigenetic modifications
• isoform sequencing (RNA Seq)
• targeted sequencing

• 30 min
• 120 min
• 240 min
• 360 min
• 480 min
• 600 min

DSMZ

Dr. Boyke Bunk
0531 2616 322
E-mail verfassen

Third generation sequencer

Sequenziermodus

Single molecule sequencing

0,5 bis 1

Laufzeit [h]

0,5 bis 6

• whole genome sequencing
• long read sequencing
• 16S rRNA gene amplicon sequencing
• epigenetic modifications
• isoform sequencing (RNA Seq)
• targeted sequencing

• 30 min
• 120 min
• 240 min
• 360 min